LOCUS       sequence-119234-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for Sp-dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: MSP712
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aacgactcag aaggggcaaa aaaacagtcg agagcggatg aagagaatag aagagggtat
       61 taaagaactg ggcagccaga tcttaaagga gcatcctgtg gaaaataccc aattgcagaa
      121 cgagaaactt tacctctatt acctacaaaa tggaagggac atgtatgttg atcaggaact
      181 ggacataaac cgtttatctg attacgacgt cgatgccatt gtaccccaat cctttttgaa
      241 ggacgattca atcgacaata aagtgcttac acgctcggat aagaaccgag ggaaaagtga
      301 caatgttcca agcgaggaag tcgtaaagaa aatgaagaac tattggcggc agctcctaaa
      361 tgcgaaactg ataacgcaaa gaaagttcga taacttaact aaagctgaga ggggtggctt
      421 gtctgaactt gacaaggccg gatttattaa acgtcagctc gtggaaaccc gccaaatcac
      481 aaagcatgtt gcacagatac tagattcccg aatgaatacg aaatacgacg agaacgataa
      541 gctgattcgg gaagtcaaag taatcacttt aaagtcaaaa ttggtgtcgg acttcagaaa
      601 ggattttcaa ttctataaag ttagggagat aaataactac caccatgcgc acgacgctta
      661 tcttaatgcc gtcgtaggga ccgcactcat taagaaatac ccgaagctag aaagtgagtt
      721 tgtgtatggt gattacaaag tttatgacgt ccgtaagatg atcgcgaaaa gcgaacag
//
