LOCUS       sequence-119240-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A carboxyl-terminal CFP fragment was fused to Gbeta-5. When 
            co-expressed with an amino terminal CFP or YFP fragment fused to a 
            Ggamma subunit or RGS7, a fluorescent signal is produced. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: CFP(159-238)-beta-5 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     275..296
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agcttggtac cgagctcgga tccgccacca tgggcaagaa cggcatcaag gcccacttca
      121 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      181 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
      241 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      301 ccgccgggat cactctcggc atggacgagc tgtacaagag atccatcgca accatggcaa
      361 ccgaggggct gcacgagaac gagacgctgg cgtcgctgaa gagcgaggcc gagagcctca
      421 agggcaagct ggaggaggag cgagccaagc tgcacgatgt ggagctgcac caggtggcgg
      481 agcgggtgga ggccctgggg cagtttgtca tgaagaccag aaggaccctc aaaggccacg
      541 ggaacaaagt cctgtgcatg gactggtgca aagataagag gaggatcgtg agctcgtcac
      601 aggatgggaa ggtgatcgtg tgggattcct tcaccacaaa caaggagcac gcggtcacca
      661 tgccctgcac gtgggtgatg gcatgtgctt atgccccatc gggatgtgcc attgcttgtg
      721 gtggtttgga taataagtgt tctgtgtacc ccttgacgtt tgacaaaaat gaaaacatgg
      781 ctgccaaaaa gaagtctgtt gctatgcaca ccaactacct
//
