LOCUS       sequence-119242-         506 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KIF1B beta aa 387-1770 with N-terminal FRB and triple myc tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Bentley M, Decker H, Luisi J, Banker G
  TITLE     A novel assay reveals preferential binding between Rabs, kinesins, 
            and specific endosomal subpopulations.
  JOURNAL   J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26.
  PUBMED    25624392
REFERENCE   2  (bases 1 to 506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2015-02-2"; volume: "208(3)"; pages: "273-281. Epub 
            2015 Jan 26"
COMMENT     Sequence Label: pBa-FRB-3myc-KIF1B beta tail 387-1770
FEATURES             Location/Qualifiers
     source          1..506
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             59..337
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 tctctgttcc tccgcagccc ccaagcttgg cgcgcctagg cctaccatgg cttctagaat
       61 cctctggcat gagatgtggc atgaaggcct ggaagaggca tctcgtttgt actttgggga
      121 aaggaacgtg aaaggcatgt ttgaggtgct ggagcccttg catgctatga tggaacgggg
      181 cccccagact ctgaaggaaa catcctttaa tcaggcctat ggtcgagatt taatggaggc
      241 ccaagagtgg tgcaggaagt acatgaaatc agggaatgtc aaggacctcc tccaagcctg
      301 ggacctctat tatcatgtgt tccgacgaat ctcaaagact agttatccgt acggaatgca
      361 gaagctgatc tcagaggagg acctgcaaaa acttatttct gaagaggatc tgcaaaagct
      421 tatttctgaa gaagatctgt acaagggagg atccggaggc natacatcca tggggtccct
      481 cacttcatcc ccgtcttcat gctcac
//
