LOCUS       sequence-119259-         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches YFP(1-158) to the N-terminus of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot
            CH
  TITLE     Visualization of G protein betagamma dimers using bimolecular 
            fluorescence complementation demonstrates roles for both beta and 
            gamma in subcellular targeting.
  JOURNAL   J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10.
  PUBMED    15136579
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-07-16"; volume: "279(29)"; pages: "30279-86. 
            Epub 2004 May 10"
COMMENT     Sequence Label: YFP(1-158) in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(37..495,496..498,499..501))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     176..195
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     435..456
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      498..507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(538..557)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(539..557)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(602..640)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 tcgagcggcc gccagtgtga tagatctctg cttgtcggcc atgatataga cgttgtggct
       61 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      121 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      181 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
      241 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc acatcaggcc
      301 gtagccgaag gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
      361 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
      421 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
      481 ctcctcgccc ttgctcacca tggtggcgga tccgagctcg gtaccaagct tgggtctccc
      541 tatagtgagt cgtattaatt tcgataagcc agtaagcagt gggttctcta gttagccaga
      601 gagctctgct tatatagacc tcccaccgta cacgcctacc g
//
