LOCUS       sequence-119260-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This G protein alpha-o construct contains internal insertions of YFP
            and the EE epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: G protein alpha-o-YFP
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattccctct
      121 cgcgctctcc ctgtctcctg tccgctccgc cgagcgatgc gagttcttgg ccccggcgac
      181 gccgcctcca gctagagatc gatctgcacc cctcaccccc ggcccggccc tctgcccagc
      241 cctgccctgc gcgcgggggt cggagaaggc gccgcggacg caccgacggc cgaggagcgg
      301 cgatgcacat gcactagcgg caccccctaa ctcactccct ccacaccccg cgccgccgcc
      361 gccgccaccg cctccgcctc cgcctcctcc tccgcctccg gcagccgcgg cagaaggacc
      421 caccctgccc cccaccccac cctccgccgg ctccggctgc ggatccagcc tcgactccta
      481 ttttatttat tttgggtcgt gcactagtct cggtgcctgc aacccgcgcc tcccgggccc
      541 gcgggcgcct cctctctcgg ctccggagcc ccagaccccg gccaccctca cctcgacacc
      601 cccagacccc agccagccgc cgctaatctt cgccgctgga atcttgatag aggctgtcct
      661 tttgggggga ttctggtctt tcgacaattt tgttcccaac caaggaaagg atatcgtgat
      721 tttctcccct ttgagcccag gctctgctct gtgggggggt ggggggcgcg c
//
