LOCUS       sequence-119263-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively active NFAT1, mutated to interfere selectively with 
            the NFAT:AP-1 interactio. Contains Puromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Martinez GJ, Pereira RM, Aijo T, Kim EY, Marangoni F, Pipkin ME, 
            Togher S, Heissmeyer V, Zhang YC, Crotty S, Lamperti ED, Ansel KM, 
            Mempel TR, Lahdesmaki H, Hogan PG, Rao A
  TITLE     The Transcription Factor NFAT Promotes Exhaustion of Activated CD8 T
            Cells.
  JOURNAL   Immunity. 2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 
            10.1016/j.immuni.2015.01.006.
  PUBMED    25680272
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 
            10.1016/j.immuni.2015.01.006."
COMMENT     Sequence Label: CA-RIT-NFAT1 (PGK-Puro)
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..26
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             111..200
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 cacaagtttg tacaaaaaag caggcttcga aggagataga accaattctc taaggaaata
       61 cttaaccatg gtcgactgga tctcgagctc aagcttccac catgatcttt tacccatacg
      121 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcctatccat
      181 atgacgttcc agattacgct gctcagtgcg gccgctctag cccggagccg cagcccgacc
      241 ccgatggcgg ggacggcccc ggccacgagc ccgggggcag tccccaagac gagctggact
      301 tttccatcct cttcgattat gactatctga accctatcga agaagaaccg atcgcacata
      361 aggccatcag ctcaccctcc ggactcgcat acccggatga tgtcctggac tatggcctca
      421 agccatgcaa cccccttgcc agtctctctg gcgagccccc tggccggttc ggagagccgg
      481 atagtatagg gttccagaac tttctgagcc cggtcaagcc agcaggggct tcgggcccga
      541 gccctcggat cgagatcact ccatcccacg aactgatgca ggcagggggg gccctccgtg
      601 ggagagacgc cggcctgtcc cccgagcagc cggccctggc cctggccggc gtggccgcca
      661 gcccgaggtt cacactgccc gtgcccggct acgagggcta ccgcgagccg ctttgcttga
      721 gccccgctgc agcaggcgca gcagccgcat tcatttctga caccttctcc cc
//
