LOCUS       sequence-119267-        9857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mNanog reporter plasmid (expresses YNL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pRZ-mNanog-YNL
FEATURES             Location/Qualifiers
     source          1..9857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1798..1914
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2556..2565
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2874..2883
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4304..4306,4307..4309,4310..4822)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(4349..4370)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4610..4629)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4957..4978
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5935..6523
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5988..6008)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6406..6417)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6614..6847
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6919..7040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6956..6975)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7010..7029
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7059..7194
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7232..7248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7232..7248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7245..7267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7256..7272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7280..7286,7287..7304,7305..7310))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7325..7346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7463..7480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7634..8222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7714..7733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8393..9184,9185..9253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9016..9035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9254..9358)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9426..9444
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9482..9504)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9604..9623
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9817..9839
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9831..9848
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9832..9848
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgat
     1921 ggtgtaaaca gtgggtctga agccttcgag ggaggagggc aagatgccgc gctctgcgtt
     1981 tctccagctg gatgtggaca gaggtcaacc agccacatta gtttatgtct aaaagtgtct
     2041 aattgaaaca agaaatggct gctttagccg ggtgtggtgg cacacgcctt taatcccagc
     2101 actcaggagg cagaggcagg cagatttctg agttcaaggc cagcctggtc tacagagtga
     2161 gttccaggac agccagagct acacagagaa accctgtctc gaaaaaccaa ccaaccaacc
     2221 aaccaaacaa acaaacaaaa aaggaaatgg ctggtttaat tatatcacac tgttcatcag
     2281 aataaagaga aaaatctcaa aaaaaaaaaa aaaaaaaaaa gaggttcagt caggctgggc
     2341 aatggaggca gtagtggccg tggtggtctt ggcagcagtg gaggccttgg tggcagtggt
     2401 gacagacagt gatagtgtgg cagcagttga ggcagtggtg gtcgtagtgg cagtgatggc
     2461 attggtggca gggttggtgt ggctcagagg tggcagtggt ggcagtggag gcattgcagg
     2521 ctttagtggc aatggtagtg gggcagcagt ggtagccatg gtggcacagt ggtagcaacg
     2581 gtggtagtgg cagccgtggt ggccgcagag gcagtggtaa caatggtggc ttggtggcag
     2641 tgctgacagt ggtagtattg gtagcattgg tggcagtgga agaagggaag tggtggtaat
     2701 ggtggcaggg gtggcagcag aggcagacag tggtgacgat ggtggcagtg gtgctgtggc
     2761 acagtggtgg cattggtggc atggcacagt ggtggcaatg atggtgtggt gcagtggtag
     2821 cagtggagac agtggtggca gtggagacag tagtagtatg gtggctgtgg tggccatggc
     2881 ggcagtggtg gcacatacag gcctttaatg ccagcacatg ggaggcagag gcagttggct
     2941 ctctgtgagt tggaagccag cttgttctac agagcaagtc ccaggacagc cagggctaca
     3001 cagagaaacc ctgcctagaa taataacaaa actcaaacaa acaccaccaa cccccgcgct
     3061 ccgccccgcc ccccccaaaa aaaaccccaa accaaaaaga gccattcaag cttagtcttt
     3121 tgtagaataa aacccaggaa gaaccactcc taccaatact cactgtggta gagtcttcac
     3181 atgggagatg ttacgagagg acggcccttc cctctctgct tatacacaga agccgactta
     3241 agctgggtta gagtgctttc actcacttat ctgtgagcac aaggactgat cggcaaactt
     3301 tgaacttggg atgtggaaat aggaagatca ggagtttggg accagctaga gcaatagact
     3361 gtgaggccag tctcgggtac gtgaaactct atctcaaaga aatgaccaaa accaaatttg
     3421 aaatacgata aatttcttct tccattgctt agacggctga ggcacttggg attgcagtaa
     3481 gtctgaagcc agcctaggtt ttacagtgag aacttgtctc aaaacaaaac agaaggcctc
     3541 tacattaatt taaacactcc ttaaattggg catggtggta gacaagcctg gtctacatac
     3601 tgagtataag ctactcaagg caacagagaa aaacctgtct caaaaccaaa gcatggacca
     3661 acttactaag gtagcccgag tcttaagcag gacacaggct ctttcttcag acttgcgtta
     3721 aaaagccgca cttttggagg gaagatttcc ccaggtttcc caatgtgaag agcaagcaag
     3781 aaacgctgag tgctgaaagg aaagccgtgt ataaacagag actttggcag caaggtctga
     3841 ctctttcatg tctgtagaaa gaatggaaga ggaaactcag atcccccact tgacctgaaa
     3901 cttcccacta gagatcgcca gggtctggag gtgcagccgt ggttaaaaga tgaataaagt
     3961 gaaatgaggt aaagcctctt tttggggggg ggggatgtct ttagatcaga ggatgccccc
     4021 taagctttcc ctccctccca gtctgggtca ccttacagct tcttttgcat tacaatgtcc
     4081 atggtggacc ctgcaggtgg gattaactgt gaattcacag ggctggtggg gcgtgggtgc
     4141 cgcctgggtg cctgggagaa tagggggtgg gtagggtagg aggcttgagg ggggaggagc
     4201 aggacctacc ctttaaatct atcgccttga gccgttggcc ttcagatagg ctgatttggt
     4261 tggtgtcttg ctctttctgt gggaaggctg cggatccacc accatggtga gcaagggcga
     4321 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4381 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4441 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg
     4501 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4561 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4621 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4681 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4741 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     4801 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
     4861 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     4921 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4981 cgccgccggg ggtaccaagg tgtacgaccc cgagcagagg aagaggatga tcaccggccc
     5041 ccagtggtgg gccaggtgca agcagatgaa cgtgctggac agcttcatca actactacga
     5101 cagcgagaag cacgccgaga acgccgtgat cttcctgcac ggcaacgcca ctagcagcta
     5161 cctgtggagg cacgtggtgc cccacatcga gcccgtggcc aggtgcatca tccccgatct
     5221 gatcggcatg ggcaagagcg gcaagagcgg caacggcagc tacaggctgc tggaccacta
     5281 caagtacctg accgcctggt tcgagcttct gaacctgccc aagaagatca tcttcgtggg
     5341 ccacgactgg ggcgccgccc tggccttcca ctacgcctac gagcaccagg acaggatcaa
     5401 ggccatcgtg cacatggaga gcgtggtgga cgtgatcgag agctgggacg agtggccaga
     5461 catcgaggag gacatcgccc tgatcaagag cgaggagggc gagaagatgg tgctggagaa
     5521 caacttcttc gtggagaccg tgctgcccag caagatcatg agaaagctgg agcccgagga
     5581 gttcgccgcc tacctggagc ccttcaagga gaagggcgag gtgagaagac ccaccctgag
     5641 ctggcccaga gagatccccc tggtgaaggg cggcaagccc gacgtggtgc agatcgtgag
     5701 aaactacaac gcctacctga gagccagcga cgacctgccc aagctgttca tcgagggcga
     5761 ccccggcttc ttcagcaacg ccatcgtgga gggcgccaag aagttcccca acaccgagtt
     5821 cgtgaaggtg aagggcctgc acttcctcca ggaggacgcc cccgacgaga tgggcaagta
     5881 catcaagagc ttcgtggaga gagtgctgaa gaacgagcag taactcgact cgacaatcaa
     5941 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6001 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6061 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6121 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6181 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6241 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6301 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6361 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6421 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgca tcttcgcctt
     6481 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattcgagc
     6541 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6601 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct
     6661 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6721 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6781 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6841 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     6901 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6961 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7021 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7081 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7141 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     7201 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7261 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7321 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7381 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7441 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7501 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7561 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7621 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7681 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7741 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7801 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7861 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7921 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7981 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8041 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8101 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8161 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8221 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8281 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8341 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8401 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8461 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8521 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8581 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8641 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8701 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8761 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8821 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8881 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8941 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9001 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9061 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9121 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9181 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9241 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9301 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9361 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9421 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     9481 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     9541 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     9601 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     9661 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     9721 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     9781 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     9841 cggccagtgc caagctg
//
