LOCUS       sequence-119268-         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A carboxyl-terminal mCerulean fragment was fused to Gbeta-5. When 
            co-expressed with an amino terminal mCerulean fragment fused to a 
            Ggamma subunit, a fluorescent signal is produced..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: Cer(159-238)-beta-5 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     287..308
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatccgccac catgggcaag aacggcatca
      121 aggcccactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
      181 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      241 gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
      301 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag agatccatcg
      361 caaccatggc aaccgagggg ctgcacgaga acgagacgct ggcgtcgctg aagagcgagg
      421 ccgagagcct caagggcaag ctggaggagg agcgagccaa gctgcacgat gtggagctgc
      481 accaggtggc ggagcgggtg gaggccctgg ggcagtttgt catgaagacc agaaggaccc
      541 tcaaaggcca cgggaacaaa gtcctgtgca tggactggtg caaagataag aggaggatcg
      601 tgagctcgtc acaggatggg aaggtgatcg tgtgggattc cttcaccaca aacaaggagc
      661 acgcggtcac catgccctgc acgtgggtga tggcatgtgc ttatgcccca tcgggatgtg
      721 ccattgcttg tgg
//
