LOCUS       sequence-119274-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ELP[V-30] in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pET-24a-ELP[V-30]
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             584..601
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(654..672)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      668..715
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagga gtacatatgc atcatcatca tcatcacagt
       61 tcagggggag taggtgtccc tggagtaggc gttccaggcg tcggggtacc tggcgtgggt
      121 gttcccggcg ttggagtccc gggtgtagga gtccccggtg ttggtgtacc tggagtggga
      181 gtaccgggag taggagtacc aggagtgggg gttcctggtg tgggagtccc aggagtcggt
      241 gtaccagggg taggggtacc gggcgtaggt gtgccaggag ttggggtgcc tggagtcgga
      301 gtccctggtg tcggagtacc tggtgttgga gtacccggag ttggtgttcc tggagttgga
      361 gttccggggg taggagttcc aggagtaggg gtcccaggtg ttggcgtacc aggtgtgggc
      421 gtgcccgggg tcggcgtccc tggggtaggt gttccaggtg taggggttcc aggggtggga
      481 gttcctgggg ttggagtgcc tggtgtaggt gtacccggct ggccgtgata atcttcagga
      541 tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca
      601 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      661 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      721 aggaggaa
//
