LOCUS       sequence-119275-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Generate OCT4 mOrange reporter line without selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Zhu Z, Verma N, Gonzalez F, Shi ZD, Huangfu D
  TITLE     A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human 
            Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 
            10.1016/j.stemcr.2015.04.016.
  PUBMED    26028531
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 
            10.1016/j.stemcr.2015.04.016."
COMMENT     Sequence Label: OCT4-2A-mOrange
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             757..813
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 actagtaacg gccgccagtg tgctggaatt cgcccttttc ctgcaggtcc cacctgcaca
       61 gatatgcaaa gcagaaaccc tcgtgcaggc ccgaaagaga aagcgaacca gtatcgagaa
      121 ccgagtgaga ggcaacctgg agaatttgtt cctgcagtgc ccgaaaccca cactgcagca
      181 gatcagccac atcgcccagc agcttgggct cgagaaggat gtgagtgcca tgtctctctg
      241 cgggctccat ctctttcccc tgtcaccacc tcgctttccc tagctctggc tcctccaact
      301 gctctagggc tgttggcttt ggacagaatg tccaagcagt caggcctgtc tcagctcatt
      361 ctctaatgtc ctcctctaac tgctctaggg ctgttggctt tggatagaat gtccaagcag
      421 agtcaggccc gtctcagctc attgtctaat gtcattctcc tttctgtcat tcacttgcag
      481 gtggtccgag tgtggttctg taaccggcgc cagaagggca agcgatcaag cagcgactat
      541 gcacaacgag aggattttga ggctgctggg tctcctttct cagggggacc agtgtccttt
      601 cctctggccc cagggcccca ttttggtacc ccaggctatg ggagccctca cttcactgca
      661 ctgtactcct cggtcccttt ccctgagggg gaagcctttc cccctgtctc cgtcaccact
      721 ctgggctctc ccatgcattc aaacgctagc accggtgcca cgaacttctc tctgttaaag
      781 caagcaggag acgtggaaga aaaccccggt cctatggtga gcaagggcga ggagaataac
      841 atggccatca tcaaggagtt catgcgcttc aaggtgcgca tggaa
//
