LOCUS       sequence-119277-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This G protein alpha-s construct contains internal insertions of YFP
            and the EE epitope..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: G protein alpha-s-YFP
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      269..278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(554..575)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg tcgaccaatt ccgcagcagc agctcccgcg gctcctgctc
      121 tgctccgcct cggccccgga gcgaggggcg gagagccgcg cgcttgcctt agtccgagcc
      181 gccaccctcc ccgcgttccc cgctttcccg gcccgcccga ggccgcccgc cccgtccccg
      241 ccgccccgca gcccggccgc gccccgccgc cgccatgggc tgcctcggca acagtaagac
      301 cgaggaccag cgcaacgagg agaaggcgca gcgcgaggcc aacaaaaaga tcgagaagca
      361 gctgcagaag gacaagcagg tctaccgggc cacgcaccgc ctgctgctgc tgggtgctgg
      421 agagtctggc aaaagcacca ttgtgaagca gatgaggatt ctacatgtta atgggtttaa
      481 cggagaggga tccggtgggg gcgggagcat ggtgagcaag ggcgaggagc tgttcaccgg
      541 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      601 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      661 cggcaagctg cccgtgccct ggc
//
