LOCUS       sequence-119283-         585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This highly effective dominant negative G protein alpha-s mutant 
            contains, in addition to the mutations in 
            alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP 
            release..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Berlot CH
  TITLE     A highly effective dominant negative alpha s construct containing 
            mutations that affect distinct functions inhibits multiple 
            Gs-coupled receptor signaling pathways.
  JOURNAL   J Biol Chem. 2002 Jun 7;277(23):21080-5. Epub 2002 Apr 1.
  PUBMED    11927592
REFERENCE   2  (bases 1 to 585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-06-7"; volume: "277(23)"; pages: "21080-5. Epub 
            2002 Apr 1"
COMMENT     Sequence Label: G protein alpha-s(alpha3beta5/G226A/A366S)
FEATURES             Location/Qualifiers
     source          1..585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      270..279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagcttg gtcgaccaat tccgcagcag cagctcccgc ggctcctgct
      121 ctgctccgcc tcggccccgg agcgaggggc ggagagccgc gcgcttgcct tagtccgagc
      181 cgccaccctc cccgcgttcc ccgctttccc ggcccgcccg aggccgcccg ccccgtcccc
      241 gccgccccgc agcccggccg cgccccgccg ccgccatggg ctgcctcggc aacagtaaga
      301 ccgaggacca gcgcaacgag gagaaggcgc agcgcgaggc caacaaaaag atcgagaagc
      361 agctgcagaa ggacaagcag gtctaccggg ccacgcaccg cctgctgctg ctgggtgctg
      421 gagagtctgg caaaagcacc attgtgaagc agatgaggat tctacatgtt aatgggttta
      481 acggagaggg cggcgaagag gacccgcagg ctgcaaggag caacagcgat ggtgagaagg
      541 ccaccaaagt gcaggacatc aaaaacaacc tgaaggaggc cattg
//
