LOCUS       sequence-119285-        5387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression hPXR mutant K62R in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5387)
  TITLE     hPXR mutants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSG5-hPXR-K62R
FEATURES             Location/Qualifiers
     source          1..5387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9..200
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(32..52)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      217..352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          395..967
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     975..994
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1022..1041
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1022..1040
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1627..1638
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2383..2504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2420..2439)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2474..2493
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2538..2555)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2709..3297)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2789..2808)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3468..4259,4260..4328))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4091..4110
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4329..4433)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4501..4519
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4558..4580)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4680..4699
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4766..5221)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4903..4924)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5115..5134
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5351..5373
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5365..5382
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5366..5382
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgacttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      301 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      361 tgcaaaaagc tggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
      421 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
      481 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
      541 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
      601 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
      661 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
      721 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
      781 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
      841 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
      901 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
      961 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1021 gtaatacgac tcactatagg gcgaattcac caccatggag gtgagaccca aagaaagctg
     1081 gaaccatgct gactttgtac actgtgagga cacagagtct gttcctggaa agcccagtgt
     1141 caacgcagat gaggaagtcg gaggtcccca aatctgccgt gtatgtgggg acaaggccac
     1201 tggctatcac ttcaatgtca tgacatgtga aggatgccgc ggctttttca ggagggccat
     1261 gaaacgcaac gcccggctga ggtgcccctt ccggaagggc gcctgcgaga tcacccggaa
     1321 gacccggcga cagtgccagg cctgccgcct gcgcaagtgc ctggagagcg gcatgaagaa
     1381 ggagatgatc atgtccgacg aggccgtgga ggagaggcgg gccttgatca agcggaagaa
     1441 aagtgaacgg acagggactc agccactggg agtgcagggg ctgacagagg agcagcggat
     1501 gatgatcagg gagctgatgg acgctcagat gaaaaccttt gacactacct tctcccattt
     1561 caagaatttc cggctgccag gggtgcttag cagtggctgc gagttgccag agtctctgca
     1621 ggccccatcg agggaagaag ctgccaagtg gagccaggtc cggaaagatc tgtgctcttt
     1681 gaaggtctct ctgcagctgc ggggggagga tggcagtgtc tggaactaca aacccccagc
     1741 cgacagtggc gggaaagaga tcttctccct gctgccccac atggctgaca tgtcaaccta
     1801 catgttcaaa ggcatcatca gctttgccaa agtcatctcc tacttcaggg acttgcccat
     1861 cgaggaccag atctccctgc tgaagggggc cgctttcgag ctgtgtcaac tgagattcaa
     1921 cacagtgttc aacgcggaga ctggaacctg ggagtgtggc cggctgtcct actgcttgga
     1981 agacactgca ggtggcttcc agcaacttct actggagccc atgctgaaat tccactacat
     2041 gctgaagaag ctgcagctgc atgaggagga gtatgtgctg atgcaggcca tctccctctt
     2101 ctccccagac cgcccaggtg tgctgcagca ccgcgtggtg gaccagctgc aggagcaatt
     2161 cgccattact ctgaagtcct acattgaatg caatcggccc cagcctgctc ataggttctt
     2221 gttcctgaag atcatggcta tgctcaccga gctccgcagc atcaatgctc agcacaccca
     2281 gcggctgctg cgcatccagg acatacaccc ctttgctacg cccctcatgc aggagttgtt
     2341 cggcatcaca ggtagctgag gatccagatc ttattaaagc agaacttgtt tattgcagct
     2401 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     2461 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggtcgact
     2521 ctagactctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2581 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2641 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2701 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2761 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2821 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2881 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     2941 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3001 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3061 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3121 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3181 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3241 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3301 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3361 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3421 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3481 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3541 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3601 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3661 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3721 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3781 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3841 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3901 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3961 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4021 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4081 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4141 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4201 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4261 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4321 atactcatac tcttcttttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4381 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4441 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     4501 aataggcgta tcacgaggcc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     4561 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     4621 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     4681 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     4741 cgtaaggaga aaataccgca tcaggaaatt gtaaacgtta atattttgtt aaaattcgcg
     4801 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     4861 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     4921 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     4981 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     5041 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     5101 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     5161 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     5221 tcgcgccatt cgccattcag gctacgcaac tgttgggaag ggcgatcggt gcgggcctct
     5281 tcgctattac gccagctggc gaagggggga tgtgctgcaa ggcgattaag ttgggtaacg
     5341 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaatt
//
