LOCUS       sequence-119288-         449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mCherry fused with out-of-frame blasticidin-S resistance 
            gene with a linker containing a target for TALEN/CRISPR. Can use as 
            a surrogate target to select cells expressing active TALEN/CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Niccheri F, Pecori R, Conticello SG
  TITLE     An efficient method to enrich for knock-out and knock-in cellular 
            clones using the CRISPR/Cas9 system.
  JOURNAL   Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y.
  PUBMED    28421278
REFERENCE   2  (bases 1 to 449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y."
COMMENT     Sequence Label: pBSR (mCherry-surrogateTarget-bsr)
FEATURES             Location/Qualifiers
     source          1..449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(118..140)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat
       61 gatggccatg ttatcctcct cgcccttgct caccatggtg aatccgctag agccgccggt
      121 cacacgccag aagccgaacc ccgccctgcc ccgtcccccc cgaaggcagc cgtccccccg
      181 cggacagccc cgaggctgga gagggagaag gggacggcgg cgcggcgacg cacgaaggcc
      241 ctccccgccc atttccttcc tgccggcgcc gcaccgcttc gccccgcgcc cgctagaggg
      301 ggtgcggcgg cgcctcccag atttcggctc cgcacagatt tgggacaaag gaagtccctg
      361 cgccctctcg cacgattacc ataaaaggca atggctgcgg ctcgccgcgc ctcgacagcc
      421 gccggcgctc cgggggccgc cgcgcccct
//
