LOCUS       sequence-119290-         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mCherry fused with out-of-frame blasticidin-S resistance 
            gene with a linker containing a target for TALEN/CRISPR. Can use as 
            a surrogate target to select cells expressing active TALEN/CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Niccheri F, Pecori R, Conticello SG
  TITLE     An efficient method to enrich for knock-out and knock-in cellular 
            clones using the CRISPR/Cas9 system.
  JOURNAL   Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y.
  PUBMED    28421278
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y."
COMMENT     Sequence Label: pBSR (mCherry-surrogateTarget-bsr)
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
       61 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      121 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      181 caagaagctt cgtctctgtc ggataatctg caactccagt ctttccagaa gatgggcggg
      241 agtcttctgg gcaaagatct gcgagacgga attctgctgg ttattgtgct gtctcatcat
      301 tttggcaaag aattaattca agcttggatt catttttgca gaaatcggag gaagaagaat
      361 atatgaaaac atttaacatt tctcaacaag atctagaatt agtagaagta gcgacagaga
      421 agattacaat gctttatgag gataataaac atcatgtggg agcggcaatt cgtacgaaaa
      481 caggagaaat catttcggca gtacatattg aagcgtatat aggacgagta actgtttgtg
      541 cagaagccat tgcgattggt agtgcagttt cgaatggaca aaaggatttt gacacgattg
      601 tagctgttag acacccttat tctgacgaag tagatagaag tattcgagtg gtaagtcctt
      661 gt
//
