LOCUS       sequence-119292-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This G protein alpha-q construct contains internal insertions of YFP
            and the EE epitope..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: G protein alpha-q-YFP
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(643..664)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct
      121 ggaattctgc agatatccat cacactggcg gccgccaccg cggtgaagct ccgcgccggc
      181 ggggctgcag cgaggcactt cggaagaatg actctggagt ccatcatggc gtgctgcctg
      241 agcgaggagg ccaaggaagc ccggaggatc aacgacgaga tcgagcggca gctgcgcagg
      301 gacaagcgcg acgcccgccg ggaactcaag ctgctgctgc tggggacagg ggagagtggc
      361 aagagcacct tcatcaagca gatgaggatc atccacgggt cgggctactc tgacgaagac
      421 aagcgcggct tcaccaagct ggtgtatcag aacatcttca cggccatgca ggccatgatc
      481 agagcgatgg acacactcaa gatcccatac aagtatgaac acaataaggc tcatgcacaa
      541 ttggttcgag aggttgatgt ggagaaggtg tctgcttttt ctggtggagg tggatccatg
      601 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      661 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      721 aagctgaccc tgaa
//
