LOCUS       sequence-119296-         329 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mCherry fused with an out-of-frame EGFP through a linker 
            containing a target for TALEN/CRISPR. Can be used as a surrogate 
            target to test the specificity of TALENs and CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Niccheri F, Pecori R, Conticello SG
  TITLE     An efficient method to enrich for knock-out and knock-in cellular 
            clones using the CRISPR/Cas9 system.
  JOURNAL   Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y.
  PUBMED    28421278
REFERENCE   2  (bases 1 to 329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y."
COMMENT     Sequence Label: pBS SK mCherryROSAegfp
FEATURES             Location/Qualifiers
     source          1..329
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(119..137)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
       61 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      121 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      181 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      241 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      301 gtgaccgtga cccaggactc ctccctgca
//
