LOCUS       sequence-119301-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression construct for targeting the murine ROSA26 locus. It
            recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK
            mCherryROSAbsr plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     rosa26 knock-in plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAGGS-ROSAf
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..84
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(181..768)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 aaaataaatt tcctttatta gccagaagtc agatgctcaa ggggcttcat gatgtcccca
       61 taatttttgg cagagggaaa aagatctcag tggtatttgt gagccagggc attggccaca
      121 ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattcctc agtcggccgc
      181 gaagttgatc tcgccgttat taaatttcct tctcacttcc tctaaggtta atgtgccggc
      241 tttaatcatt tctccaccaa ttaaaagctc ttctacacta agaacagctc cattacaatt
      301 agtgatatga tttaatcgtg taagctgagc tttgtagttt cctttaaagt gaccactcac
      361 aaataaaaac ttaaattccg ttacagaaga tggatagact ttccaccatt cattagggtt
      421 gatatgtttg tttcgtgttt gattttcttc gacatatcgt tgcatttcat ctgcttggcc
      481 aattggcaga ttataacctc cgctataagc tttagtatcc acgatcacac cgtaatcaat
      541 aggagatccg acagtataaa ttgctccgtc cggtttcctt gatccaccca aatgtttacc
      601 tctatatcca taaactttca taaaaaattc cattaccttc atttcaagaa ttctatcctg
      661 agtggaattt ctggcaattt caattaattc aatatattca tgaggcacat atttcaattt
      721 atgacgaagt tcagatttct tctcctccag ttcacttttg actagctggg atctgatcaa
//
