LOCUS       sequence-119303-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf6 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARF6-GFP
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(624..648)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(680..699)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(681..699)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(744..948)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(773..793)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttgcta gccattctag atcgaaccac
       61 tttgtacaag aaagctgggt cagatttgta gttagaggtt aaccatgtga gcccctcata
      121 gagtccgtcc cctgaggtgg cacaggaggg ctgcacatac cagttcctgt cccgaatccg
      181 ggtcaggccc agtttctcct ggatctcgtg gggtttcatg gcatcgggca ggtcctgctt
      241 gttggcgaag atgaggatta tggcgtccct catctcccgg tcattgataa tgcggtgcag
      301 ctcctggcga gcctcatcga tgcggtcgcg gtcggcgcag tccactacga agatgagacc
      361 ttgggtccca gtgtagtaat gccgccagag cggccggatc ttgtcctggc cgcccacatc
      421 ccatacgttg aacttgacat ttttgtaagt caccgtctcc acgttgaaac ccacagtggg
      481 aatggtggtc accgactggc ccagcttcaa cttgtacagg attgttgtct tgccggccgc
      541 gtccaggccc aacatgagga tccgcatttc cttgttcccg aagattttgg atagcacctt
      601 ccccatggtt ctatctcctt ctaagcctgc ttttttgtac aaacttgttt gatcaagctt
      661 aactagccag cttgggtctc cctatagtga gtcgtattaa tttcgataag ccagtaagca
      721 gtgggttctc tagttagcca gagagctctg cttatataga cctcccaccg tacacgccta
      781 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
      841 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa atccccgtga
      901 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
      961 gatgactaa
//
