LOCUS       sequence-119305-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G protein alpha-s internally tagged with ECFP and EE epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH
  TITLE     Live cell imaging of Gs and the beta2-adrenergic receptor 
            demonstrates that both alphas and beta1gamma7 internalize upon 
            stimulation and exhibit similar trafficking patterns that differ 
            from that of the beta2-adrenergic receptor.
  JOURNAL   J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.
  PUBMED    15297467
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-15"; volume: "279(42)"; pages: "44101-12. 
            Epub 2004 Aug 5"
COMMENT     Sequence Label: G protein alpha-s-CFP
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      272..281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(557..578)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtcgacca attccgcagc agcagctccc gcggctcctg
      121 ctctgctccg cctcggcccc ggagcgaggg gcggagagcc gcgcgcttgc cttagtccga
      181 gccgccaccc tccccgcgtt ccccgctttc ccggcccgcc cgaggccgcc cgccccgtcc
      241 ccgccgcccc gcagcccggc cgcgccccgc cgccgccatg ggctgcctcg gcaacagtaa
      301 gaccgaggac cagcgcaacg aggagaaggc gcagcgcgag gccaacaaaa agatcgagaa
      361 gcagctgcag aaggacaagc aggtctaccg ggccacgcac cgcctgctgc tgctgggtgc
      421 tggagagtct ggcaaaagca ccattgtgaa gcagatgagg attctacatg ttaatgggtt
      481 taacggagag ggatccggtg ggggcgggag catggtgagc aagggcgagg agctgttcac
      541 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      601 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      661 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
      721 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttctt
//
