LOCUS       sequence-119307-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl1 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL1-GFP
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(641..665)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(697..716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(698..716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(761..964)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(790..810)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attgggacaa ctccagtgaa aagttcttct cctttgctag ccattctaga tcgaaccact
       61 ttgtacaaga aagctgggtc ctgtctgctt tttaatgttt caactaacca ttccattgcc
      121 tcatcaaggc cggtgccttt ggttgctgac gttttgaata tctgccattt tcggtccttc
      181 aaggcaggta acccaagtga atttgccatc tctgaggaag tcatggcctg ttccatgtcc
      241 tgtttatttg caaacaccac taaaatggct tttctcagct cttcttcctc caacatggca
      301 actaactctg atttggaaat gccaattcgg tctcggtcac aactgtctac tacataaatg
      361 actgcatctg tgtttgaata gtaacatctc caatatggcc tgatacttgt ctgtcctcct
      421 aaatcccaga cttggaattt aaggtttttg tacgtcaccg tctctacatt aaatccaatg
      481 gtaggtatag tagtaacaac ttctcccact tgtaatctgt acaaaattgt ggtttttcct
      541 gctccatcta atcccaaaat taaaattctc atttcccgag ttccaaacag actggaaaat
      601 atacttgaga aaaagccacc catggttcta tctccttcta agcctgcttt tttgtacaaa
      661 cttgtttgat caagcttaac tagccagctt gggtctccct atagtgagtc gtattaattt
      721 cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct
      781 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
      841 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
      901 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
      961 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaag
//
