LOCUS       sequence-119308-         212 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mCherry fused with out-of-frame blasticidin-S resistance 
            gene with a linker containing a target for TALEN/CRISPR. Can use as 
            a surrogate target to select cells expressing active TALEN/CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Niccheri F, Pecori R, Conticello SG
  TITLE     An efficient method to enrich for knock-out and knock-in cellular 
            clones using the CRISPR/Cas9 system.
  JOURNAL   Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y.
  PUBMED    28421278
REFERENCE   2  (bases 1 to 212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y."
COMMENT     Sequence Label: pBS SK mCherryROSAbsr
FEATURES             Location/Qualifiers
     source          1..212
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(122..140)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
       61 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccanacc
      121 gccaagctga aggtgaccaa gggtggcccc ctgcccttcn cctgggacat cctgtcccct
      181 cagttcatgt acggctccna ggcctacgtg aa
//
