LOCUS       sequence-119312-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In addition to the mutations in alpha-s(alpha3beta5), this dominant 
            negative G protein alpha-s mutant contains the G226A mutation, which
            impairs activating conformation changes in Switch II..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Berlot CH
  TITLE     A highly effective dominant negative alpha s construct containing 
            mutations that affect distinct functions inhibits multiple 
            Gs-coupled receptor signaling pathways.
  JOURNAL   J Biol Chem. 2002 Jun 7;277(23):21080-5. Epub 2002 Apr 1.
  PUBMED    11927592
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-06-7"; volume: "277(23)"; pages: "21080-5. Epub 
            2002 Apr 1"
COMMENT     Sequence Label: G protein alpha-s(alpha3beta5/G226A)
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      271..280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtcgaccaa ttccgcagca gcagctcccg cggctcctgc
      121 tctgctccgc ctcggccccg gagcgagggg cggagagccg cgcgcttgcc ttagtccgag
      181 ccgccaccct ccccgcgttc cccgctttcc cggcccgccc gaggccgccc gccccgtccc
      241 cgccgccccg cagcccggcc gcgccccgcc gccgccatgg gctgcctcgg caacagtaag
      301 accgaggacc agcgcaacga ggagaaggcg cagcgcgagg ccaacaaaaa gatcgagaag
      361 cagctgcaga aggacaagca ggtctaccgg gccacgcacc gcctgctgct gctgggtgct
      421 ggagagtctg gcaaaagcac cattgtgaag cagatgagga ttctacatgt taatgggttt
      481 aacggagagg gcggcgaaga ggacccgcag gctgcaagga gcaacagcga tggtgagaag
      541 gccaccaaag tgcaggacat caaaaacaac ctgaaggagg ccattgaaac cattgtggcc
      601 gccatgagca acctggtgcc ccccgtggag ctggccaacc ctgagaacca gttcagagtg
      661 gactacattc tgagcgtgat gaacgtgcca aactttgact tcccacctga attctatgag
      721 catgccaagg ctctgtggg
//
