LOCUS       sequence-119313-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PDX1-eGFP mini-vector donor for integration of the eGFP reporter 
            into the PDX1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Zhu Z, Verma N, Gonzalez F, Shi ZD, Huangfu D
  TITLE     A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human 
            Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 
            10.1016/j.stemcr.2015.04.016.
  PUBMED    26028531
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 
            10.1016/j.stemcr.2015.04.016."
COMMENT     Sequence Label: PDX1-2A-eGFP
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(620..641)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cacgcggtgg cggccgctct agaactcact gtattccact ggcatcaatt tcacgggatc
       61 tttgaaaact caaacatttc ctttcaatta tttctaaact taaaggggtc gcccgagtaa
      121 gaatggcttt atggcagatt aaggtactcg gcccagcttc cccgctgtgt gtgttaggga
      181 gccttccaat gtgtatggta cagtttccat tgtctttgaa gagccggctt ctctaaacag
      241 gtcccaaggt ggagtgctgt aggagggcag ggatgtgcgc aacagccaca aacaacgcca
      301 atccagtatc actaaaagag ggccccaata ctacaaaaac caatggctct ttttcttccg
      361 gaaggcctgc cggcatctac ccgcggggcc ccaactggca catgcgcctg agagagcggg
      421 ttttcccctt cggtctaagg tgggccccgc aattgccgcc ccattcaact gccagggcgg
      481 gtggtccacg cccggggtcc tcctaggccc tctgctcctc ctcggcgagt ggttgaagcc
      541 cctcagccag gagcagctcc tgcctcggcg cgccttactt gtacagctcg tccatgccga
      601 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
      661 ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
      721 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
      781 tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat
      841 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcc
//
