LOCUS       sequence-119316-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PDX1-eGFP mini-vector donor for integration of the eGFP reporter 
            into the PDX1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Zhu Z, Verma N, Gonzalez F, Shi ZD, Huangfu D
  TITLE     A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human 
            Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 
            10.1016/j.stemcr.2015.04.016.
  PUBMED    26028531
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 
            10.1016/j.stemcr.2015.04.016."
COMMENT     Sequence Label: PDX1-2A-eGFP
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             730..786
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(832..853)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gaccactcat tggcagagcc aagcttaggc tcacggcgag agctgactcg agtttggtct
       61 ccaataaaaa ggctatcttt attaggaagg gcttgagtta ctagggaaga gcttcgcgcg
      121 cctacactag gcgctgaaat gggatgctgg ggcttggtgg ctccggcggg agcagctggt
      181 agggctaggg ctccctggcc ccccttgaag cggttgggct gcgtgggtgg gggctgtgcg
      241 gggctccggg ggccacactc acgccctgtg tcgcccgcag gcggcgccta cgctgcggag
      301 ccggaggaga acaagcggac gcgcacggcc tacacgcgcg cacagctgct agagctggag
      361 aaggagttcc tattcaacaa gtacatctca cggccgcgcc gggtggagct ggctgtcatg
      421 ttgaacttga ccgagagaca catcaagatc tggttccaaa accgccgcat gaagtggaaa
      481 aaggaggagg acaagaagcg cggcggcggg acagctgtcg ggggtggcgg ggtcgcggag
      541 cctgagcagg actgcgccgt gacctccggc gaggagcttc tggcgctgcc gccgccgccg
      601 ccccccggag gtgctgtgcc gcccgctgcc cccgttgccg cccgagaggg ccgcctgccg
      661 cctggcctta gcgcgtcgcc acagccctcc agcgtcgcgc ctcggcggcc gcaggaacca
      721 cgagctagcg ccactaactt ctccctgttg aaacaagcag gggatgtcga agagaatccc
      781 gggccaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      841 ctggacggcg acgtaa
//
