LOCUS       sequence-119322-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mCherry fused with out-of-frame blasticidin-S resistance 
            gene with a linker containing a target for TALEN/CRISPR. Can use as 
            a surrogate target to select cells expressing active TALEN/CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Niccheri F, Pecori R, Conticello SG
  TITLE     An efficient method to enrich for knock-out and knock-in cellular 
            clones using the CRISPR/Cas9 system.
  JOURNAL   Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y.
  PUBMED    28421278
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y."
COMMENT     Sequence Label: pBS SK mCherryROSAbsr
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
       61 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      121 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
      181 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
      241 aagcttcgtc tctgtcggat aatctgcaac tccagtcttt ccagaagatg ggcgggagtc
      301 ttctgggcaa agatctgcga gacggaattc tgctggttat tgtgctgtct catcattttg
      361 gcaaagaatt aattcaagct tggattcatt tttgcagaaa tcggaggaag aagaatatat
      421 gaaaacattt aacatttctc aacaagatct agaattagta gaagtagcga cagagaagat
      481 tacaatgctt tatgaggata ataaacatca tgtgggagcg gcaattcgta cgaaaacagg
      541 agaaatcatt tcggcagtac atattgaagc gtatatagga cgagtaactg tttgtgcaga
      601 agccattgcg attggtagtg cagtttcgaa tggacaaaag gattttgaca cgattgtagc
      661 tgttagacac ccttattctg acgaagtaga tagaagtatt cgagtggtaa gtccttgtgg
      721 tatgtgtagg gagttgattt cagactatgc accagattgt tttgtgttaa tagaaatgaa
      781 tggcaagtta gtcaaaacta cgattgaaga actcattcca ctcaaatata cccga
//
