LOCUS       sequence-119323-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mCherry fused with out-of-frame blasticidin-S resistance 
            gene with a linker containing a target for TALEN/CRISPR. Can use as 
            a surrogate target to select cells expressing active TALEN/CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Niccheri F, Pecori R, Conticello SG
  TITLE     An efficient method to enrich for knock-out and knock-in cellular 
            clones using the CRISPR/Cas9 system.
  JOURNAL   Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y.
  PUBMED    28421278
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y."
COMMENT     Sequence Label: pBS SK mCherryROSAbsr
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(422..442)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          572..637
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
       61 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctctagtc aaggcactat
      121 acatcaaata ttccttatta acccctttac aaattaaaaa gctaaaggta cacaattttt
      181 gagcatagtt attaatagca gacactctat gcctgtgtgg agtaagaaaa aacagtatgt
      241 tatgattata actgttatgc ctacttataa aggttacaga atatttttcc ataattttct
      301 tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc aaagcaagca agagttctat
      361 tactaaacac agcatgactc aaaaaactta gcaattctga aggaaagtcc ttggggtctt
      421 ctacctttct cttctttttt ggaggagtag aatgttgaga gtcagcagta gcctcatcat
      481 cactagatgg catttcttct gagcaaaaca ggttttcctc attaaaggca ttccaccact
      541 gctcccattc atcagttcca taggttggaa tctaaaatac acaaacaatt agaatcagta
      601 gtttaacaca ttatacactt aaaaatttta tatttacctt agagctttaa atctctgtag
      661 gtagtttgtc caattatgtc acaccacaga agtaaggttc cttcacaaag atccggacca
      721 aagcggccat cgtgcctccc cactcctgca gttcgggggc atggatgcgc ggatagccgc
      781 tgctggtttc ctggatgccg acggatttgc actgccggt
//
