LOCUS       sequence-119324-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression construct for targeting the murine ROSA26 locus. It
            recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK
            mCherryROSAbsr plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  TITLE     rosa26 knock-in plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAGGS-ROSAr
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(176..763)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
       61 tttggcagag ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc
      121 caccaccttc tgataggcag cctgcacctg aggagtgaat tcctcagtcg gccgcgaagt
      181 tgatctcgcc gttattaaat ttccttctca cttcctctaa ggttaatgtg ccggctttaa
      241 tcatttctcc accaattaaa agctcttcta cactaagaac agctccatta caattagtga
      301 tatgatttaa tcgtgtaagc tgagctttgt agtttccttt aaagtgacca ctcacaaata
      361 aaaacttaaa ttccgttaca gaagatggat agactttcca ccattcatta gggttgatat
      421 gtttgtttcg tgtttgattt tcttcgacat atcgttgcat ttcatctgct tggccaattg
      481 gcagattata acctccgcta taagctttag tatccacgat cacaccgtaa tcaataggag
      541 atccgacagt ataaattgct ccgtccggtt tccttgatcc acccaaatgt ttacctctat
      601 atccataaac tttcataaaa aattccatta ccttcatttc aagaattcta tcctgagtgg
      661 aatttctggc aatttcaatt aattcaatat attcatgagg cacatatttc aatttatgac
      721 gaagttcaga tttcttctcc tccagttcac ttttgactag ctgggatctg atcaaattcc
      781 ggcgcgtgcg gcaatccctt tttcactgca tccatggc
//
