LOCUS       sequence-119325-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vanillate-inducible gene expression plasmid for Sphingomonas melonis
            Fr1; destination vector for Gateway cloning, allowing C-terminal 
            fusion of proteins to HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Synthetic vanillate-regulated promoter for graded gene expression in
            Sphingomonas.
  JOURNAL   Sci Rep. 2014 Sep 29;4:6453. doi: 10.1038/srep06453.
  PUBMED    25262659
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-09-29"; volume: "4:6453. doi"; pages: " 
            10.1038/srep06453"
COMMENT     Sequence Label: pVHD
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(103..109,110..127,128..133))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(158..282)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(301..327)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
      121 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
      181 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
      241 atcattttac gtttctcgtt cagctttttt gtacaaactt gtctctagag tcatattaat
      301 ggcgtagtcc gggacgtcgt acgggtacat gcggaagctt cctctactag tattggatcc
      361 aatttagcgg cttcgcggcg atccgtcaag attggatcca atgttaactc cggtacattg
      421 acatatgtat ccgctcatga tacaataacc ctgatgctag cagggagaga ccccgaatgg
      481 acatgccgcg cataaagccg ggccagcgcg tgatgatggc tcttcgcaag atgatcgcct
      541 cgggcgagat caagagcggc gagcgtatcg ccgaaatccc gacagcggcg gcgctgggcg
      601 tctcgcgcat gcccgtgcgc acggccttgc ggagtctgga gcaggaaggg ctcgtggtgc
      661 ggctgggcgc gcggggttat gccgcccgag gcgtgtccag cgaccagatc cgcgacgcca
      721 tcgaggtgcg aggggtgctg gagggctttg cggcccggcg tctggccgag cgcggaatga
      781 cggcggagac ccacgcgcgg ttcgtcgcct tgatcgccga gggcgaagcg ctgttcgcgg
      841 ctgggcggct taacggcgag gacctggatc gctacgccgc gtacaatcag gccttccatg
      901 acacgctggt cagcgctgcg ggcaacggcg ctgtcgagag cgccctggcg cgcaatggtt
      961 tcgagccgtt cgcggcggcc ggtgcgctgg ccctggatct gatggacttg ccggccgagt
     1021 acgaacacct tctggccgcc catcgccagc atcaggcggt gctgga
//
