LOCUS       sequence-119326-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vanillate-inducible gene expression plasmid for Sphingomonas melonis
            Fr1; destination vector for Gateway cloning, allowing C-terminal 
            fusion of proteins to HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Synthetic vanillate-regulated promoter for graded gene expression in
            Sphingomonas.
  JOURNAL   Sci Rep. 2014 Sep 29;4:6453. doi: 10.1038/srep06453.
  PUBMED    25262659
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-09-29"; volume: "4:6453. doi"; pages: " 
            10.1038/srep06453"
COMMENT     Sequence Label: pVHD
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(118..242)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             284..349
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(421..438)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(421..437)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(430..452)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(646..665)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     765..787
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(825..843)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
       61 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat
      121 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa
      181 tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg
      241 gtgatatcgc tagctagagg taccgagctc caattgggat ccggactaca aggaccacga
      301 cggcgactat aaggaccacg acatcgacta caaggatgac gacgacaagt gaggatccgg
      361 tgaagtgacc cgcaaaaaaa aaggccaggg gatcgctccc ccggcctttt ttttgaattc
      421 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      481 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      541 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
      601 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
      661 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      721 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
      781 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
      841 atttttatag gttaatgtca tgataataat ggtttcttag caccctttct cggtccttca
      901 acgttcctga caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc
      961 tcgaacgctg cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc
     1021 gagagcggag cctgttcaac ggtgccgccg cgctcgccgg catcgct
//
