LOCUS       sequence-119327-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vanillate-inducible gene expression plasmid for Sphingomonas melonis
            Fr1; destination vector for Gateway cloning, allowing C-terminal 
            fusion of proteins to HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Synthetic vanillate-regulated promoter for graded gene expression in
            Sphingomonas.
  JOURNAL   Sci Rep. 2014 Sep 29;4:6453. doi: 10.1038/srep06453.
  PUBMED    25262659
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-09-29"; volume: "4:6453. doi"; pages: " 
            10.1038/srep06453"
COMMENT     Sequence Label: pVHD
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      158..746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     647..666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
       61 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      181 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      241 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      301 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      361 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      421 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      481 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      541 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      601 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      661 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      721 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      781 ccttttgctg gccttttgct cacatgtttt cagtcggcgc gaatgctcca cgccgcgccc
      841 agcggcgcgc cggcgctcgc cgcggcctcg aagaccttgg cgttacgaat ggcggccagg
      901 gcatggtcgc gcatgatccg ctcggccccc tcggcgtcgc cgcacgagac cgcgtccagc
      961 accgcctgat gctggcgatg ggcggccaga aggtgttcgt actcggccgg caagtccatc
     1021 agatccaggg
//
