LOCUS       sequence-119338-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This G protein alpha-s mutant exhibits increased affinity for and 
            decreased ability to be activated by Gs-protein-coupled receptors as
            well as decreased ability to activate adenylyl cyclase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Berlot CH
  TITLE     A highly effective dominant negative alpha s construct containing 
            mutations that affect distinct functions inhibits multiple 
            Gs-coupled receptor signaling pathways.
  JOURNAL   J Biol Chem. 2002 Jun 7;277(23):21080-5. Epub 2002 Apr 1.
  PUBMED    11927592
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-06-7"; volume: "277(23)"; pages: "21080-5. Epub 
            2002 Apr 1"
COMMENT     Sequence Label: G protein alpha-s(alpha3beta5)
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      272..281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtcgacca attccgcagc agcagctccc gcggctcctg
      121 ctctgctccg cctcggcccc ggagcgaggg gcggagagcc gcgcgcttgc cttagtccga
      181 gccgccaccc tccccgcgtt ccccgctttc ccggcccgcc cgaggccgcc cgccccgtcc
      241 ccgccgcccc gcagcccggc cgcgccccgc cgccgccatg ggctgcctcg gcaacagtaa
      301 gaccgaggac cagcgcaacg aggagaaggc gcagcgcgag gccaacaaaa agatcgagaa
      361 gcagctgcag aaggacaagc aggtctaccg ggccacgcac cgcctgctgc tgctgggtgc
      421 tggagagtct ggcaaaagca ccattgtgaa gcagatgagg attctacatg ttaatgggtt
      481 taacggagag ggcggcgaag aggacccgca ggctgcaagg agcaacagcg atggtgagaa
      541 ggccaccaaa gtgcaggaca tcaaaaacaa cctgaaggag gccattgaaa ccattgtggc
      601 cgccatgagc aacctggtgc cccccgtgga gctggccaac cctgagaacc agttcagagt
      661 ggactacatt ctgagcgtga tgaacgtgcc aaactttgac ttcccacctg aattctatga
      721 gcatgccaag gctctgtggg aggatgaggg agttcgtgcc tgctacgagc gctccaacga
      781 gtaccag
//
