LOCUS       sequence-119341-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A C-terminal CFP fragment was fused to Ggamma-2. When co-expressed 
            with an N-terminal mCerulean, CFP, or YFP fragment fused to a Gbeta 
            subunit with which it interacts a fluorescent signal is produced..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: CFP(159-238)-gamma-2 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     291..312
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(626..644)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(627..644)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatccg ccaccatggg caagaacggc
      121 atcaaggccc acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      181 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      241 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      301 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagagatcc
      361 atggccagca acaacaccgc cagcatagca caagccagga aactggtaga acagctgaag
      421 atggaagcca acatcgatag gataaaggtg tccaaggcag ctgcagattt gatggcctac
      481 tgtgaagcgc atgccaagga agatcccctc ctgacacctg ttccggcttc agaaaaccca
      541 tttagggaga agaagttttt ctgtgctatc ctttaaagat ctatcacact ggcggccgct
      601 cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga ggatctttgt
      661 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
      721 ctctaaaggt aaatata
//
