LOCUS       sequence-119342-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches YFP(159-238) to the N-terminus of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot
            CH
  TITLE     Visualization of G protein betagamma dimers using bimolecular 
            fluorescence complementation demonstrates roles for both beta and 
            gamma in subcellular targeting.
  JOURNAL   J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10.
  PUBMED    15136579
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-07-16"; volume: "279(29)"; pages: "30279-86. 
            Epub 2004 May 10"
COMMENT     Sequence Label: YFP(159-238) in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     288..309
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(401..419)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(402..419)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          503..568
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccgcca ccatgggcaa gaacggcatc
      121 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      181 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      241 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      301 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gagatctatc
      361 acactggcgg ccgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg
      421 ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
      481 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
      541 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
      601 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
      661 tgatgaggct actgctgact ctcaaca
//
