LOCUS       sequence-119343-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches YFP(159-238) to the N-terminus of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot
            CH
  TITLE     Visualization of G protein betagamma dimers using bimolecular 
            fluorescence complementation demonstrates roles for both beta and 
            gamma in subcellular targeting.
  JOURNAL   J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10.
  PUBMED    15136579
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-07-16"; volume: "279(29)"; pages: "30279-86. 
            Epub 2004 May 10"
COMMENT     Sequence Label: YFP(159-238) in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(71..92)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      274..283
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(314..333)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(315..333)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(378..581)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(407..427)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcgagcggc cgccagtgtg atagatctct tgtacagctc gtccatgccg agagtgatcc
       61 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
      121 tcagggcgga ctggtagctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
      181 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
      241 ggatcttgaa gttcaccttg atgccgttct tgcccatggt ggcggatccg agctcggtac
      301 caagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta agcagtgggt
      361 tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg cctaccgccc
      421 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
      481 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
      541 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
      601 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
      661 caggcgggcc atttaccgtc attgacgtca ata
//
