LOCUS       sequence-119344-         587 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches YFP(159-238) to the C-terminus of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 587)
  TITLE     Unpublished BiFC Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: YFP(159-238)Stop in pcDNA1/amp
FEATURES             Location/Qualifiers
     source          1..587
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     276..297
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(392..410)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(393..410)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          494..559
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccaaga acggcatcaa ggtgaacttc
      121 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      181 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
      241 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      301 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagatctat cacactggcg
      361 gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat gctagaggat
      421 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
      481 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
      541 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatggg
//
