LOCUS       sequence-119346-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of fluorescent membranes in sea urchin primordial germ 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Campanale JP, Gokirmak T, Espinoza JA, Oulhen N, Wessel GM, Hamdoun 
            A
  TITLE     Migration of sea urchin primordial germ cells.
  JOURNAL   Dev Dyn. 2014 Jul;243(7):917-27. doi: 10.1002/dvdy.24133. Epub 2014 
            Apr 30.
  PUBMED    24677545
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.24133"; journalName: "Dev Dyn"; date: "2014-07"; 
            volume: "243"; issue: "7"; pages: "917-27"
COMMENT     Sequence Label: CmCitrine Lck w/ NosUTRs
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(303..322)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(304..322)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(329..346)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(329..345)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(338..360)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      486..866
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(573..592)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     783..804
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctcaccatgg tggcgaccgg tgcttcatca tcattcaaat tatcttttcg ttttgattta
       61 atacaaccca tggcggcctc gtcgtcgttc aggttgtcct tgcgcttgct cttgatgcag
      121 cccatggatc cggtgactaa agtgcgtgga aactcgagac agtaaatttt gtaaagagcg
      181 tagtacgtcg tagagctagt caagaatgat ctaaattgaa tttgagaaag tattgcaatt
      241 tatccacatg tgcaacaaac tcatttatgt gggctttaat gaggcacgtg gggcccaatt
      301 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg
      361 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      421 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      481 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      541 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      601 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      661 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      721 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      781 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
      841 tttataaggg attttgccga tttcggccta ttggttaaaa
//
