LOCUS       sequence-119347-         518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adenovirus for the expression of gRNAs targeting intron 19 of murine
            Alk and intron 14 of Eml4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC,
            Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura
            A
  TITLE     In vivo engineering of oncogenic chromosomal rearrangements with the
            CRISPR/Cas9 system.
  JOURNAL   Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 
            2014 Oct 22.
  PUBMED    25337876
REFERENCE   2  (bases 1 to 518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13902"; journalName: "Nature"; date: "2014-12-18- 
            18"; volume: "516"; issue: "7531"; pages: "423-7"
COMMENT     Sequence Label: Adeno EA
FEATURES             Location/Qualifiers
     source          1..518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(148..223)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(245..493)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(303..322)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(473..493)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacggg
       61 taccccattt gtctgcagaa ttggcgcacg cgctaaaaac ggactagcct tattttaact
      121 tgctatttct agctctaaaa caaaaaagca ccgactcggt gccacttttt caagttgata
      181 acggactagc cttattttaa cttgctattt ctagctctaa aactcctagt agaccccgac
      241 aaacggtgtt tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag
      301 ttacggtaag catatgatag tccattttaa aacataattt taaaactgca aactacccaa
      361 gaaattatta ctttctacgt cacgnatttt gtactaatat ctttgtgttt acagtcaaat
      421 taattccaat tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg
      481 gaaataggcc ctctctanag ccatttgtct gcagaatt
//
