LOCUS       sequence-119361-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  hSara with stop codon in Gateway Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDONR221-SARA
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(731..755)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gtcctattaa
      121 tcaatgtact gtgccatcca gcggaagcct tctccgtaac cttgtctttt gagcacacta
      181 cacatgaaaa cttctaaggg tcgggcattc agttctttca gagatatact cccctttcct
      241 gttgtctgac catataaacc aaacatctct cgcaacctct cttcactgat ggcttcaggt
      301 ctgtcgatct tattcccaag aatcagtata ggcacattag caatggtttc atctgtcatt
      361 agtgaatcaa gttcttcttt tgactctaac agcctttcgt ggtctgcaca atccaccaga
      421 aatacaatgc cattgatagc aggaaggtag tttttccaca ctcttcgagc ttgaacatgt
      481 ccacccagat caaaagttgt aaacgtcatg ccagcaatgg tcagttcttc ggaagtggga
      541 tgtaatgttg ggacatgttg tccaagtctg tcatctttta gcatgtgtag caatgttgtt
      601 tttcctgcat tatccaatcc aagaaatacc agtttaccag ttttcttata taatcctaaa
      661 aactgtagca cactgctgaa accactgtaa atccaatcaa atatgaagga catggttcta
      721 tctccttcta agcctgcttt tttgtacaaa gttggcatta taaaaaagca ttgctcatca
      781 atttgttgca acgaacaggt cactatcagt caaaaataaa atcattattt ggggcccg
//
