LOCUS       sequence-119366-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..52
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             56..76
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             80..100
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             113..790
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     514..533
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     708..731
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tgaaccgtca gatccgctag ccaccatgga tccaaaaaag aagagaaagg tagatccaaa
       61 aaagaagaga aaggtagatc caaaaaagaa gagaaaggta ccggtggcta ccatggtgag
      121 caagggcgag gaggtcatca aggagttcat gcgcttcaag gagcacatgg agggctccgt
      181 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      241 gaccgccagg ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
      301 ccctcagatc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
      361 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      421 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
      481 gaaggtgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
      541 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatgaa
      601 gatgaggctg aggctgaagg acggcggcca ctacgacgcc gaggtcaaga ccacctacat
      661 ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc tggacatcac
      721 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg gccgccactc
      781 caccggcgcc tccggagaag ggcaagggca agggcaaggg ccgggccgcg gctacgcgta
      841 tcgatccatg ttcgaggcgc gcctggtcca gggctccatc ctcaagaagg tgttggaggc
      901 actcaaggac ctcatcaacg aggcctg
//
