LOCUS       sequence-119372-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf4 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARF4-Myc
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(646..670)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(781..799)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(845..896)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(874..894)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtcac gttttgaaag ctcatttgac agccagtcaa
      121 gtccttcata cagaccagtt ccttgtgttg cacaagtggc ttgaacatac catgttctgt
      181 tacgaagaga ctgaagccct agtttatctg tcatttcact gatggcaata gcatttggca
      241 aatcctgttt gtttgcaaaa ggtagcagca ctgcatctct caattcatct accagaagca
      301 ttttctgcag ctcatctgct acttcctgaa ttctttcacg atcgttgcta tctaccacaa
      361 aaataagacc ctgggtattc tggaagtaat gcttccagag aggcctaatt ctatcttgac
      421 caccaacatc ccatactgtg aaacaaatgt tcttatattc tactgtttcc gcattaaaac
      481 caatggtagg aatggtggtg actatctccc ctaacttcag tttatacaga atggttgtct
      541 tgccagcagc atccaatcca accatcaaaa tgcgcatctg cttcttgcca aatagtcggg
      601 agaagaggga ggagatagtg aggcccatgg ttctatctcc ttctaagcct gcttttttgt
      661 acaaacttgt ttgattcgag tgtcgacggt atcgataagc ttgatatcga attcctgcag
      721 cccgggggat ccgcccgggc tagagcggcc gccaccgcgg tggagctcca gcttttgttc
      781 cctttagtga gggttaattt cgagcttggc gtaatcgcta gcggatctga cggttcacta
      841 aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
      901 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
      961 ccattgacgt ca
//
