LOCUS       sequence-119376-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf6 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARF6-Myc
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(631..655)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(766..784)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(830..929)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(859..879)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtcag atttgtagtt agaggttaac catgtgagcc
      121 cctcatagag tccgtcccct gaggtggcac aggagggctg cacataccag ttcctgtccc
      181 gaatccgggt caggcccagt ttctcctgga tctcgtgggg tttcatggca tcgggcaggt
      241 cctgcttgtt ggcgaagatg aggattatgg cgtccctcat ctcccggtca ttgataatgc
      301 ggtgcagctc ctggcgagcc tcatcgatgc ggtcgcggtc ggcgcagtcc actacgaaga
      361 tgagaccttg ggtcccagtg tagtaatgcc gccagagcgg ccggatcttg tcctggccgc
      421 ccacatccca tacgttgaac ttgacatttt tgtaagtcac cgtctccacg ttgaaaccca
      481 cagtgggaat ggtggtcacc gactggccca gcttcaactt gtacaggatt gttgtcttgc
      541 cggccgcgtc caggcccaac atgaggatcc gcatttcctt gttcccgaag attttggata
      601 gcaccttccc catggttcta tctccttcta agcctgcttt tttgtacaaa cttgtttgat
      661 tcgagtgtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccgcc
      721 cgggctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt
      781 aatttcgagc ttggcgtaat cgctagcgga tctgacggtt cactaaacca gctctgctta
      841 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
      901 gacattttgg aaagtcccgt tgattttggt gccaaaacaa aactcccatt gacgtcaatg
      961 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgta
//
