LOCUS       sequence-119378-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf5 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARF5-Myc
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(646..670)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(781..799)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(845..944)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(874..894)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtcgc gctttgacag ctcgtgggac agccagtcca
      121 gaccatcgta cagacctgtg ccttgggtgg cacaggtggc ctggacatac cacgtgcggc
      181 tgcgtaagtg ctgtagcccc agcttgtcag tcagctcgct cacgggcatg gcgttgggca
      241 tgtcctgctt gttggcaaat accagcagca ctgcatcccg cagctcgtcc tcctgcagca
      301 tcttctggag ttcatcagca gattcttgga cccgctcccg gtcattactg tccaccacaa
      361 agatgaggcc ctgagtgttc tggaagtagt gccgccacag aggccgaatc ttgtcctggc
      421 ctcccacgtc ccagactgtg aaacagatgt tcttatattc cactgtttct acattgaagc
      481 ctatggttgg gatggtggtg acaatctccc ccaacttcag tttgtacagg attgtggtct
      541 tgccagccgc atccaagcca accatgagaa tccgcatctg cttcttcccg aagatccgcg
      601 aaaagagcgc ggacacggtg aggcccatgg ttctatctcc ttctaagcct gcttttttgt
      661 acaaacttgt ttgattcgag tgtcgacggt atcgataagc ttgatatcga attcctgcag
      721 cccgggggat ccgcccgggc tagagcggcc gccaccgcgg tggagctcca gcttttgttc
      781 cctttagtga gggttaattt cgagcttggc gtaatcgcta gcggatctga cggttcacta
      841 aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
      901 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
      961 cattgacgtc aatgggggtg ga
//
