LOCUS       sequence-119381-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl2 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARL2-Myc
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(658..682)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(793..811)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(857..908)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(886..906)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtcgt cagctgtgaa aatgcggctg gaaatgtcat
      121 ccaggagcca gtcgatgccc ggcagcaggt tctccccggt gacggcgctg cagccctgga
      181 tgcaccagtg gtggctgcgg atggagtcca gctccagggc ctcgcggatg gcgttagagg
      241 acagtgctcc aggcaggtcc tgcttattag caaagatgag gagggttgct ccggccaggc
      301 gctcctccac cagcaggctc tggagctccc gctggcagtc ctgcatgcgc tggcggtctg
      361 cgctgtccac tacccagatg aggccatcgg tgctctcaaa gtagttccgc cagtaggacc
      421 gcagggactt ctggccaccc acatcccaga tgttcagctt gaatcctcgg tgctccaggg
      481 tcttgatgtt gaagcccagc gttggggaga tggtgtcgat gtcctcccca ttgaacttct
      541 tcaggatggt tgtctttcca gcattgtcca ggccaagcat gagcagtcgc agctcccgct
      601 ctttctgctt catcttcttc agaatggtca ggagccccat ggttctatct ccttctaagc
      661 ctgctttttt gtacaaactt gtttgattcg agtgtcgacg gtatcgataa gcttgatatc
      721 gaattcctgc agcccggggg atccgcccgg gctagagcgg ccgccaccgc ggtggagctc
      781 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcgc tagcggatct
      841 gacggttcac taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
      901 tttgcgtcaa tggg
//
