LOCUS       sequence-119382-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl3 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARL3-Myc
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(651..675)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(786..804)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(850..901)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(879..899)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctggtcttt cttctttgca ttgacatttt tgcagaccca
      121 gttcatgcca tcctgaacgc cctctcctgt gagagctgag caagactgga tctgccagac
      181 tcggtcgcgg atggtatgca ggttcagtcc ttctgcaatt tcagaggcag gggctgctgt
      241 gagcaaatcc tgcttattag caaagatgag cactggcaca caacttagtt tttcttcctc
      301 cagtaattcc gctagttcct gacccgtctc ttcaaatctt tttctgtctg cactgtcgat
      361 tacatatata agaatatcgg tattttcaaa ataattcttc cagtatggtc tgattttcct
      421 ctgtccacca atgtcccata cattcagttt aaaaccttgt gattgtacac ttttgatgtt
      481 gaaaccctgt gtaggtgtga tgtggctgat gtcttcagat gcaagctgct tcagaagagt
      541 ggtcttgcca gcattatcca agcccaggag aagtattctc acctcctggt ctggtgcact
      601 tttcaacttg cgcaaaattg agagcaagcc catggttcta tctccttcta agcctgcttt
      661 tttgtacaaa cttgtttgat tcgagtgtcg acggtatcga taagcttgat atcgaattcc
      721 tgcagcccgg gggatccgcc cgggctagag cggccgccac cgcggtggag ctccagcttt
      781 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat cgctagcgga tctgacggtt
      841 cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
      901 caatggggcg gagttgt
//
