LOCUS       sequence-119386-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Macrolide biosensor. Low copy plasmid that expresses GFP 
            proportional to macrolide concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-L-mphR
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             611..619
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gagatccagc actagttatt acgcgtgcgg ctgcagcagc tggaagtcgt cgtgttccgg
       61 gaacatcagg cacaggatcg ccgcgatctg cgccagaacg tggtccgcca gttcaccgtc
      121 cgggtcaacc gcccactgca tggtcgcacc cgcgataaca gagtgcagca gcagttccgc
      181 cgccgccggc gcacccggcg gcagacgttt acggatacct tcaacaaccg cacggttacg
      241 ctggatcgcc agggtacgca gttccggaac ctgcagttcg taccaagaga tcaggtagtt
      301 aacagagaag tcgttacggg tgttcataga acgaaccaga acctgcagga attcccacag
      361 accctgcgga cccgcaccga tcgggatcgc gttcaggtag tgacgaacct gttcaacacc
      421 acgttccatc atacgaacca gcagggtgtc acggttggtg aaacgctgga tcagcgccgc
      481 acgagacaga ccaacttctt tcgcaacacc agacagggtg aattcgatcg gaccgcaacg
      541 tttcagaaca acggtcgccg cttccagaac ttcgtcgtca gatttcagtt tcggacgcgg
      601 catttgtatt acctccttat ttcaaagtta aacaaaatta tttgtagagg gaaaccgttg
      661 tggtctccct gaatatagcc tacgagcctt atgcatgccc gtaaagttat ccagcaacca
      721 ctcatagacc tagggcagca gatagggacg acgtggtgtt agctgtggta aacgccatgg
      781 gcattaaaa
//
