LOCUS       sequence-119389-         549 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose biosensor. High copy plasmid that expresses GFP 
            proportional to arabinose concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-araC
FEATURES             Location/Qualifiers
     source          1..549
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(294..459)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(352..371)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gtggtccggg taacgagcga agcactgaac accgtaggtc agggtggtaa ccagggtcgg
       61 ccacggaacc ggcagtttac cggtggtgca gatgaatttc agggtcagtt taccgttggt
      121 agcgtcacct tcaccttcac cacgaacaga gaatttgtga ccgttaacgt caccgtccag
      181 ttcaaccagg atcggaacaa caccggtgaa cagttcttca cctttacgca tatgtatatc
      241 tccttcttaa agttaaagtt agcccaaaaa aagtgaaaga tatgaaagcg ggtatggaga
      301 aacagtagag agttgcgata aaaagcgtca ggtaggatcc gctaatctta tggataaaaa
      361 tgctatggca tagcaaagtg tgacgccgtg caaataatca atgtggactt ttctgccgtg
      421 attatagaca cttttgttac gcgtttttgt catggctttg gtcccgcttt gttacagaat
      481 gcttttaata agcggggtta ccggttgggt tagcgagaag agccagtaaa agacgcagtg
      541 acggcaatg
//
