LOCUS       sequence-119397-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl5 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARL5A-Myc
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(643..667)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(778..796)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(842..893)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(871..891)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtctc taatcttaag tcgtgacatc atccattcaa
      121 gtccttggca caatccctcg ccagttagag cacagcatgc ctggatatgc cactggtgat
      181 ctttaataga agttagcttc aaaaactggg agatttctgc tacagtcatg cattctttaa
      241 catcttgttt attagcaaaa atcagcaatc cagcttttct taggtcctca tgcgctaaca
      301 ttttatagag ttcttctcta gttacagaaa tcctctctct gtctgtactg tccacaacaa
      361 ctattacaaa ctctgtgtta gtatagtaag tgttccagga agaacgaaga gattcttggc
      421 caccaatatc ccacattagg aaacgtgtat tattaatcac tatctcttct acattacttc
      481 ctattgtagg agatgtatgt acaacttcat tcatagaaaa ttggtaaaga atggtagttt
      541 tccctgcatt atccaaccca acaatgataa ctttgtgctc ctggtgattg aacagtctcc
      601 atattctagt gaagagaatt cccatggttc tatctccttc taagcctgct tttttgtaca
      661 aacttgtttg attcgagtgt cgacggtatc gataagcttg atatcgaatt cctgcagccc
      721 gggggatccg cccgggctag agcggccgcc accgcggtgg agctccagct tttgttccct
      781 ttagtgaggg ttaatttcga gcttggcgta atcgctagcg gatctgacgg ttcactaaac
      841 cagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
      901 cggagttgtt acgacatttt ggaaagtccg gttgat
//
