LOCUS       sequence-119398-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl5b with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARL5B-Myc
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(643..667)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(778..796)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(842..941)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     promoter        complement(845..1042)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(871..891)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtctc tcacaccaat ccgggaggtc atccactcta
      121 gaccttggca taacccttct cctgtgagag cacagcagga ttgaatgtgc catggatgat
      181 ccttaattga actaagggtg aggtatttcg agatttcagc tgctgtcata caccctttca
      241 tatcctgttt atttgcaaag ataaggactg cagccttccg taaatcctca tgagccaaca
      301 ttctgtataa ttcttctttt gtaatagcta gtcgttccct gtcaatgcta tcaacaacaa
      361 gaatgatgaa ctctgtattt gagtaatatg tgttccagga tgatcgcaga gactcctgac
      421 caccaatatc ccacataaga aaatgagtgt tcttcacaac tatttcttca acattgcttc
      481 ctatggttgg agaagtatga accacttcat tcattaagaa ttggtaaaga atggtggttt
      541 tccctgcatt atccagtccc actataatta ctttgtgttc ttggttacag aagaggctcc
      601 acagtttggc gaagatcagc cccatggttc tatctccttc taagcctgct tttttgtaca
      661 aacttgtttg attcgagtgt cgacggtatc gataagcttg atatcgaatt cctgcagccc
      721 gggggatccg cccgggctag agcggccgcc accgcggtgg agctccagct tttgttccct
      781 ttagtgaggg ttaatttcga gcttggcgta atcgctagcg gatctgacgg ttcactaaac
      841 cagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
      901 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
      961 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     1021 tgatgtactg ccaaaccgca tccccatggt aatagcgatg actaat
//
