LOCUS       sequence-119399-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the fbiC gene from Mycobacterium smegmatis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Selby CP, Sancar A
  TITLE     The second chromophore in Drosophila photolyase/cryptochrome family 
            photoreceptors.
  JOURNAL   Biochemistry. 2012 Jan 10;51(1):167-71. doi: 10.1021/bi201536w. Epub
            2011 Dec 27.
  PUBMED    22175817
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi201536w";
            journalName: "Biochemistry"; date: "2012-01-10- 10"; volume: "51"; 
            issue: "1"; pages: "167-71"
COMMENT     Sequence Label: pfbiRS
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..78)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     124..143
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        124..142
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    143..167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             223..240
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 aattaatacg actcactata ggggaattgt gagcggataa caattcccct gtagaaataa
      181 ttttgtttaa ctttaataag gagatatacc atgggcagca gccatcacca tcatcaccac
      241 agccaggatc cgaattcgaa ctccgagcac ggcgccgatc tgggtgtccc actggtccct
      301 ccgcccgccg taacgccgag tcctgcagct ctgcgacgtg tattgcggcg agcccgcgac
      361 ggtgtcgccc tgaacgtgga tgaagccgcc atcgcgatga cggcacgcgg cgacgatctc
      421 gccgacctgt gtgccagcgc ggcccgggtg cgcgacgccg ggttgaccac ggcggggcgg
      481 cgcggtccga acggcaggct gccggtgagc tactcgcgca aggtgttcat cccggtgaca
      541 catctgtgcc gcgacacgtg ccactactgc acgttcgtga ccgtgcccgg caagctgcgc
      601 gcgcagggca tgggcatgta catggagcct gacgagatcc tggacgtcgc ccgtcaagga
      661 gctgaactgg gttgcaaaga ggcccttttc acgctcggcg accgccccga ggaccggtgg
      721 gaggaagccc gccagtggct ggacgagcgt ggttacgact ccacgctgga ctacgtccgg
      781 gccatggcga tccgcgtgct cgaagagacc ggcctgctgc cgcatctcaa tcccggcgtc
      841 atgagctggt cggagctgtc gcggctcaag ccggtcgcgc cgtcgatggg catgatgctg
      901 gagaccacgt cgcggcgcct gttcgagacc aaaggcgaag cgcactacgg cagcccggac
      961 aaggatcccg cggtgcgcct gcgcacgctc gacgacgcgg gacggctgtc catcccgttc
     1021 accacgggac tgctggtggg catcggtgag acgctg
//
